Standard Operating Procedures for Biospecimen Collection, Processing, and Storage
Year: 2018
Type: article
Abstract: High-quality and well-annotated biorepositories are needed to better understand the pathophysiology and biologic mechanisms of chronic pancreatitis (CP) and its consequences. We report a methodology for the development of a robust standard operating procedure (SOP) for a biorepository based on the experience of the clinical centers within the consortium to study Chronic Pancreatitis, Diabetes and Pancreas Cancer Clinical Centers (CPDPC), supported by the National Cancer Institute and the National Institute for Diabetes and Digestive and Kidney Diseases as a unique multidisciplinary model to study CP, diabetes, and pancreatic cancer in both children and adults. Standard operating procedures from the CPDPC centers were evaluated and consolidated. The literature was reviewed for standard biorepository operating procedures that facilitated downstream molecular analysis. The existing literature on biobanking practices was harmonized with the SOPs from the clinical centers to produce a biorepository for pancreatic research. This article reports the methods and basic principles behind the creation of SOPs to develop a biorepository for the CPDPC. These will serve as a guide for investigators developing biorepositories in pancreas research. Rigorous and meticulous adherence to standardized biospecimen collection will facilitate investigations to better understand the pathophysiology and biologic mechanisms of CP, diabetes, and pancreatic cancer.
Source: Pancreas
Authors: William E. Fisher, Zobeida Cruz-Monserrate, Amy L. McElhany, Gregory B. Lesinski, Phil A. Hart, Ria Ghosh, George Van Buren, Douglas S. Fishman, Jo Ann S. Rinaudo, Jose Serrano, Sudhir Srivastava, Thomas Mace, Mark Topazian, Ziding Feng, Dhiraj Yadav, Stephen J. Pandol, Steven J. Hughes, Robert Y. Liu, Emily Lu, Robert Orr, David C. Whitcomb, Amer S. Abouhamze, Hanno Steen, Zachary M. Sellers, David M. Troendle, Aliye Uc, Mark E. Lowe, Darwin L. Conwell
Institutions: Baylor College of Medicine, Dan L Duncan Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, The Ohio State University, Emory University, Winship Cancer Institute, The University of Texas MD Anderson Cancer Center, National Cancer Institute, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, Mayo Clinic, University of Pittsburgh, Cedars-Sinai Medical Center, University of Florida Health, University of Florida, Indiana Clinical and Translational Sciences Institute, Boston Children's Hospital, Harvard University, Lucile Packard Children's Hospital, Stanford University, The University of Texas Southwestern Medical Center, University of Iowa Stead Family Children’s Hospital, Washington University in St. Louis
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1390620369, G1454999011, G2434943017, G336615015, G3613016580, The Ohio State University Chronic Pancreatitis Diabetes Pancreas Cancer (CPDPC) Clinical Center, G3921619115, G631332572, The Stanford Clinical Center for the Chronic Pancreatitis Research Consortium (CPCRC), UPMC Clinical Center for the Study of Chronic Pancreatitis and Diabetes, Cedars Sinai Center for the Chronic Pancreatitis Clinical Research Consortium, INSPPIRE: Using the natural history of pediatric chronic pancreatitis to identify disease predictors and design therapies, Southern California Research Center for ALPD and Cirrhosis, Precision Medicine Center for Cystic Fibrosis, The Exocrine and Endocrine Pancreas in Type 2 Diabetes, Pancreatitis and Cancer
OpenAlex ID: W2896422686