Overexpression of Bcl-3 sustains MMP-1 expression against inhibition by CDDO in SW-1353 cells
Year: 2011
Type: other
Abstract: Copyright information:Taken from "The triterpenoid CDDO inhibits expression of matrix metalloproteinase-1, matrix metalloproteinase-13 and Bcl-3 in primary human chondrocytes"Arthritis Research & Therapy 2003;5(5):R285-R291.Published online 8 Jul 2003http://www.ncbi.nlm.nih.gov/pmc/articles/PMC193729.Copyright © 2003 Elliott et al., licensee BioMed Central Ltd (Print ISSN 1478-6354; Online ISSN 1478-6362). This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. SW-1353 cells, stably transfected with a pBkRSV vector with or without a Bcl-3 insert, were incubated for 24 hours with 300 nM CDDO before the addition of 10 ng/ml IL-1. After a further 18 hours of culture, RNA was harvested, reverse transcribed and subjected to quantitative real-time PCR for MMP-1 and MMP-13 . Results were normalized to GAPDH, and are means of culture triplicates. They are expressed as molecules per molecule of GAPDH. Two-tailed Student's -tests were performed on the data, and significance is indicated as follows: *< 0.1; **0.01
Source: Figshare
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2896430928