Design & Validation of a Hybrid-capture Next-generation Sequencing-based T-cell Clonality Assay
Year: 2016
Type: dissertation
Abstract: This project details the development of a novel NGS-based T-cell clonality assay, incorporating all four TcR loci. The assay was both analytically and clinically validated. For the former, a series of idealized specimens was used, with combined PCR/Electrophoresis and Sanger Sequencing to confirm NGS-data. The latter validation compared NGS results to the current gold standard for clinical T-cell clonality testing (i.e. the BIOMED primer PCR-based method) on an appropriately-sized minimally-biased sample of hematopathology specimens. In the latter dataset, the patterns of T-cell clonality were also correlated with clinical, pathologic, and outcome data.
Source: TSpace (University of Toronto)
Author: Etienne Mahé
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2896584238