Work

Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase

Year: 2018

Type: article

Abstract: , the Superfamily 1 (Sf1) helicase Srs2, which is an ATP hydrolysis-dependent motor protein that actively dismantles recombination intermediates, promotes synthesis-dependent strand annealing, the result of which is a reduction in cross-over recombination products. Here, we show that the meiosis-specific recombinase Dmc1 is a potent inhibitor of Srs2. Biochemical and single-molecule assays demonstrate that Dmc1 acts by inhibiting Srs2 ATP hydrolysis activity, which prevents the motor protein from undergoing ATP hydrolysis-dependent translocation on Dmc1-bound recombination intermediates. We propose a model in which Dmc1 helps contribute to cross-over formation during meiosis by antagonizing the antirecombinase activity of Srs2.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1810457115

Authors: J. Brooks Crickard, Kyle Kaniecki, Youngho Kwon, Patrick Sung, Eric C. Greene

Institutions: Columbia University, Yale University

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green