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Strand transfer (target capture) assay

Year: 2011

Type: other

Abstract: Copyright information:Taken from "A bifunctional DNA binding region in Tn transposase"Molecular Microbiology 2007;67(3):528-540.Published online 16 Dec 2007PMCID:PMC2229646.© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd The strand transfer reaction generates two products: single-end strand transfer (SEST) product (open-circle DNA) and double-end strand transfer (DEST) product (linear DNA). The amount of DEST product (in fluorescence units, FUs) was determined after 24 h. In comparison with the control, six mutant Tnps are hypoactive for strand transfer: R104A, K160A, K164A, R189C, R210A and H213A.

Source: Figshare

DOI: 10.6084/m9.figshare.21477

Authors: Gradman, Richard J, Ptacin, Jerod L, Bhasin, Archna, Reznikoff, William S, Goryshin, Igor Y

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2896906773

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