Work

Motion-resolved quantitative phase imaging

Year: 2018

Type: article

Abstract: The temporal resolution of quantitative phase imaging with Differential Phase Contrast (DPC) is limited by the requirement for multiple illumination-encoded measurements. This inhibits imaging of fast-moving samples. We present a computational approach to model and correct for non-rigid sample motion during the DPC acquisition in order to improve temporal resolution to that of a single-shot method and enable imaging of motion dynamics at the framerate of the sensor. Our method relies on the addition of a simultaneously-acquired color-multiplexed reference signal to enable non-rigid registration of measurements prior to phase retrieval. We show experimental results where we reduce motion blur from fast-moving live biological samples.

Source: Biomedical Optics Express

DOI: 10.1364/boe.9.005456

Authors: Michael Kellman, Michael Chen, Zachary F. Phillips, Michael Lustig, Laura Waller

Institution: University of California, Berkeley

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: National Science Foundation, Gordon and Betty Moore Foundation