Work

Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway

Year: 2018

Type: article

Abstract: High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.

Source: eLife

DOI: 10.7554/elife.40444

Authors: Nicholas Chim, Lynnette N Jackson, Anh M Trinh, John C Chaput

Institution: University of California, Irvine

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,500