Work

Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

Year: 2018

Type: article

Abstract: Introduction: Helicobacter pylori infection induces clinical symptoms in 15-20% of subjects, and the reason for this variation is still not clear.The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic H. pylori infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms.Material and methods: Ninety subjects (aged 35-49 years) were enrolled in the study and separated into 3 groups of 30 subjects each.Group I -healthy volunteers without H. pylori infection, group II -subjects with asymptomatic H. pylori infection, group III -H.pylori-infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain.To diagnose H. pylori infection the urea breath test (UBT-13C) and histological analysis were performed.The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR.Results: The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II (p > 0.05) and 4.40 ±1.64 in group III (p < 0.001).The levels of expression of TpH-1 in gastric body mucosa were 2.16 ±0.70, 1.57 ±0.52 (p > 0.05) and 3.40 ±1.51 (p < 0.001), respectively.In group III a positive correlation was found between intensity of H. pylori colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms.Conclusions: Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia.

Source: Archives of Medical Science

DOI: 10.5114/aoms.2018.76928

Authors: Cezary Chojnacki, Tomasz Popławski, Aleksandra Błońska, Janusz Błasiak, Marek Romanowski, Jan Chojnacki

Institutions: Medical University of Lodz, University of Łódź

Language: English

Topic: Helicobacter pylori-related gastroenterology studies

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,078