Work

CRISPR/Cas9 can mediate high-efficiency off-target mutations in mice in vivo

Year: 2018

Type: article

Abstract: The CRISPR/Cas system has revolutionized the field of genome-editing as it is easier, faster, less expensive, and more efficient than traditional methods. In a very short time, its applications have become very diverse and widely used. Recently, this technology has been applied in human embryos to correct pathogenic mutations and has re-ignited the ethical debate of germ-line editing 1 , 2 . Besides the ethical considerations, the possibility of creating off-target mutations with unknown consequences is a concern.

Source: Cell Death and Disease

DOI: 10.1038/s41419-018-1146-0

Authors: Neeraj K Aryal, Amanda R Wasylishen, Guillermina Lozano

Institutions: The University of Texas MD Anderson Cancer Center, The University of Texas Health Science Center at Houston

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $4,990

Funders: University of Texas MD Anderson Cancer Center, National Cancer Institute