Studies of human hemoglobin modified with peroxynitrite: A cytotoxic metabolite generated in numerous disorders.
Year: 2018
Type: article
Abstract: OBJECTIVES: . The native and modified Hbs were characterized using various biochemical methods. METHODS: The native and modified Hbs were characterized using absorption spectroscopy, thermal melting profile analysis, and other biochemical techniques. We have also tried to ascertain the effect of various scavengers such as uric acid, ascorbic acid, tocopherol, and reduced glutathione as potent peroxynitrite quenchers. RESULTS: The isolated Hb produces distinct peaks while the Hb modified with peroxynitrite showed marked hyperchromicity and the distinct peaks were lost. The chemical denaturation and thermal denaturation studies along with carbonyl content data show that the modified Hb is unstable and shows higher absorbance due to denaturation of the protein. CONCLUSION: studies presented here show that peroxynitrite is toxic to human Hb and its inhibition by various antioxidants may be helpful in prevention of numerous disorders.
Source: Europe PMC (PubMed Central)
Authors: Purva Agarwal, Haseeb Ahsan, Rizwan Ahmad
Institutions: Jamia Millia Islamia, Imam Abdulrahman Bin Faisal University
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2899278707