Year: 2017
Type: dissertation
Abstract: Alpha thalassemia/mental retardation syndrome X-linked (ATRX) is linked to both developmental disease and cancer. ATRX functions in coordination with Death Domain Associated Protein 6 (DAXX) in a histone deposition pathway of a histone 3 variant at heterochromatin. While ATRX has recently been implicated in many alternative functions, mechanistic information is lacking. We performed proximity dependent biotinylation and protein identification to determine an unbiased list of labile ATRX interactors. As expected, we found transcriptional repressors and DAXX. Surprisingly, we also found many proteins involved in homologous recombination and nucleotide excision repair to be interacting with ATRX in the absence of DNA damaging events. Additional interactions include proteins involved in centromere maintenance and one nucleolar protein involved in RNA processing. These results suggest ATRX has a dynamic role in DNA damage repair and provide further support for its role as a nuclear protein involved in H3.3 deposition at endogenous retroviral elements.
Source: TSpace (University of Toronto)
Author: S. Jonathon
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2899613450