Year: 2018
Type: article
Abstract: Class switch recombination (CSR) in B cells involves deletion-recombination at switch (S) region DNA and is important for the diversification of antibody isotypes during an immune response. Here, we identify two NME [NM23/NDPK (nucleoside diphosphate kinase)] isoforms, NME1 and NME2, as novel players in this process. Knockdown of NME2 leads to decreased CSR, while knockdown of the highly homologous NME1 results in increased CSR. Interestingly, these NME proteins also display differential occupancy at S regions during CSR despite their homology; NME1 binds to S regions prior to stimulation, while NME2 binds to S regions only after stimulation. To the best of our knowledge, this represents the first report of a role for these proteins in the regulation of CSR.
Source: FEBS Letters
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,980
Awards: Cellular/Molecular Basis of Development: Research Center, Mechanistic Elucidation of Class Switch Recombination and Somatic Hypermutation, G4102770861, Cellular/Molecular Basis of Development: Research Center, G9000003249, Cancer Center Support Grant, RNA-directed targeting of AID in immunity and cancer, Mechanistic Elucidation of Class Switch Recombination and Somatic Hypermutation, 2/2 CCNY-MSKCC Partnership for Cancer Research, Education and Community Outreach, 1/2 CCNY-MSK Partnership for Cancer Research, Education and Community Outreach
OpenAlex ID: W2900362496