Year: 2015
Type: article
Abstract: This protocol describes a method to graft conventional primed pluripotent stem cells \(PSCs) and regionselective PSCs to different locations of post-implantation mouse epiblast followed by _in vitro_ embryo culture.Compared to conventionally used teratoma formation assay, epiblast grafting offers a more relevant _in vivo_ test for evaluating the primed PSCs' pluripotency and uniquely serves to distinguish different avors of primed PSCs that harbor distinct spatial properties.Moreover, the observation that grafted human rsESCs could enter into early mouse embryogenesis provides a novel way of studying human development.
Source: Protocol Exchange
Institutions: Salk Institute for Biological Studies, Kindai University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2900743603