Ribosome-induced RNA conformational changes in a viral 3′-UTR sense and regulate translation levels
Year: 2018
Type: article
Abstract: Structured RNA elements, programmed RNA conformational changes, and interactions between different RNA domains underlie many modes of regulating gene expression, mandating studies to understand the foundational principles that govern these phenomena. Exploring the structured 3' untranslated region (UTR) of a viral RNA, we discovered that different contexts of the 3'-UTR confer different abilities to enhance translation of an associated open reading frame. In one context, ribosome-induced conformational changes in a 'sensor' RNA domain affect a separate RNA 'functional' domain, altering translation efficiency. The structure of the entire 3'-UTR reveals that structurally distinct domains use a spine of continuously stacked bases and a strut-like linker to create a conduit for communication within the higher-order architecture. Thus, this 3'-UTR RNA illustrates how RNA can use programmed conformational changes to sense the translation status of an upstream open reading frame, then create a tuned functional response by communicating that information to other RNA elements.
Source: Nature Communications
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Awards: G1286236842, G1286251239, G1414775356, G1445641779, G1816284028, Macromolecular X-ray Data Collection Instrumentation, G2812968099, G30714459, G3083819904, Non-coding RNA Structure through a Mutate-and-Map Strategy, G365575497, G382641700, Manipulation of cellular translation machinery by viral RNAs, G419881185, G42916606, G4501827968, G498139845, G5849692847, G650090426, G6652958823, G805243471, Structure, function, and dynamics of viral RNAs and RNA-containing complexes, University of Colorado Cancer Center Support Grant, G969889393
OpenAlex ID: W2900983837