Work

Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

Year: 2018

Type: article

Abstract: A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.

Source: Organic & Biomolecular Chemistry

DOI: 10.1039/c8ob02467g

Authors: Neeraj N. Patwardhan, Zhengguo Cai, Colby N. Newson, Amanda E. Hargrove

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Duke University, National Institute of General Medical Sciences

Awards: The Center for HIV RNA Studies (CRNA), Harnessing Small Molecules to Probe the Structure and Function of Regulatory RNAs, G755304650

OpenAlex ID: W2901950296

FWCI: 1.689

Cites: 66

Cited by: 36

Related to: 10