Work

Pooled extracellular receptor-ligand interaction screening using CRISPR activation

Year: 2018

Type: article

Abstract: Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.

Source: Genome biology

DOI: 10.1186/s13059-018-1581-3

Authors: Zheng-Shan Chong, Shuhei Ohnishi, Kosuke Yusa, Gavin J. Wright

Institution: Wellcome Sanger Institute

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $5,490