Work

Spatially encoded polarization-dependent nonlinear optics

Year: 2018

Type: article

Abstract: A single fixed optic is combined with the sample translation capabilities inherent to most microscopes to achieve precise polarization-dependent second harmonic generation microscopy measurements of thin tissue sections. Although polarization measurements have enabled detailed structural analysis of collagen, challenges in integrating rotation stages or fast electro-optic/photoelastic modulation have complicated the retrofitting of existing systems for precise polarization analysis. Placing a static microretarder array in the rear conjugate plane resulted in spatially encoded polarization modulation across the field of view. A complete set of polarization rotation measurements was acquired at each pixel by sample translation, recovering local-frame tensors relating to structure within collagenous tissue.

Source: Optics Letters

DOI: 10.1364/ol.43.005973

Authors: James R. W. Ulcickas, Changqin Ding, Fengyuan Deng, Garth J. Simpson

Institution: Purdue University West Lafayette

Language: English

Topic: Optical Polarization and Ellipsometry

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute of General Medical Sciences

Awards: GOALI: CDS&E: Sensitively Quantifying Trace Crystallinity and Crystal Form within Pharmaceutical Formulations, Nonlinear Optical Imaging for Guiding Protein Structure Determination