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Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy

Year: 2018
Type: preprint
Abstract: In utero electroporation is increasingly used for manipulation of gene expression in the mammalian central nervous system. Here we created a transposon-based single plasmid system, that allows long-term conditional loss- and gain-of-function experiments across major CNS cell types. The system is widely applicable across species, allowing fast, flexible and cheap cell manipulation in previously non-transgenic species.
DOI: 10.1101/481580
Authors: Michal Slezak, Filip de Vin, Yohei Shinmyo, Mykhailo Y. Batiuk, Melvin Y. Rincon, Carmen Menacho Pando, Johann Urschitz, Stefan Moisyadi, Frank Schnütgen, Hiroshi Kawasaki, Matthew G. Holt
Institutions: VIB-KU Leuven Center for Brain & Disease Research, KU Leuven, Kanazawa University, University of Hawaiʻi at Mānoa, University of Hawaii System, Goethe University Frankfurt, University Hospital Frankfurt, Frankfurt Cancer Institute
Language: English
Subfield: Genetics
Open Access status: green
Funders: Kanazawa University, Deutsche Forschungsgemeinschaft, Ministry of Education, Culture, Sports, Science and Technology, Fonds Wetenschappelijk Onderzoek, National Institutes of Health, Japan Society for the Promotion of Science, FP7 Ideas: European Research Council
Awards: Plastic circuits: in vivo imaging of astrocyte-neuron interactions in the mouse brain, Role/s of CYFIP1 in brain development and implications in neurodevelopmental disorders., Single cell heterogeneity of hippocampal astrocytes: investigating molecular diversity and its correlation to functional specialization., G479012064, G7459681254, Molecular Studies of Astrocyte Function in Health and Disease, 児童・生徒の知的能力の構造とその発達的変化に関する分析的研究, G9000126235
OpenAlex ID: W2903318684
Cites: 19
Cited by: 2
Related to: 10