Work

Rapid Determination of Antibiotic Susceptibility by Stimulated Raman Scattering Imaging of D 2 O Metabolism

Year: 2018

Type: preprint

Abstract: Rapid antibiotic susceptibility testing (AST) is urgently needed for treating infections with correct antibiotics and slowing down the emergence of antibiotic resistant bacteria. Current clinical methods reply on culture and take at least 16 h. Here, using P. aeruginosa , E. coli and S. aureus as models, we show that the AST can be finished in 10 minutes by stimulated Raman scattering (SRS) imaging of D 2 O metabolic activities. The metabolic incorporation of D 2 O, which is used for biomolecule synthesis, can be monitored in a single bacterium. Time lapse experiments show that the C-D vibrational signal can be observed in a single bacterium within 10 minutes culture in D 2 O medium. Since water is universally used for biosynthesis in bacteria, SRS imaging of D 2 O metabolism has the potential to be generalizable to different bacteria species.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/496778

Authors: Weili Hong, Ji-Xin Cheng

Institutions: Beihang University, Boston University

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: W. M. Keck Foundation