Combinatorial Peptide Microarray Synthesis Based on Microfluidic Impact Printing
Year: 2018
Type: article
Abstract: In this Research Article, a novel inkjet printing technique, micro impact printing (MI printing), is applied for the first time to combinatorial peptide microarray synthesis on amine functionalized microdisc arrays through standard Fmoc chemistry. MI printing shows great advantages in combinatorial peptide microarray synthesis compared with other printing techniques, including (1) a disposable cartridge; (2) a small spot size (80 μm) increases array density; (3) minimal loading volume (0.6 μL) and dead volume (<0.1 μL), reduce chemical waste; and (4) multiplexibility of 5 channels/cartridge and capacity of multiple cartridges. Using this synthesis platform, a tetrapeptide library with 625 permutations was constructed and then applied for the screening of ligands targeting α4β1 integrin on Jurkat cells.
Source: ACS Combinatorial Science
Institutions: University of California, Davis, Tufts University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: G1308682533, IDBR: Encoded Combinatorial Microdisc Array for Ultrahigh-Throughput Biomolecule Screening, Digital one-disc-one-compound array for high-throughput discovery of cancer-targe, G2366971526, G4232970317, CAREER: Lab-on-a-Chip Systems of Photopatternable Multifunctional Nanocomposite Materials for Cell Detection and Manipulation, G5238372043, G5672407639, G723867762, G7367588204, G776903742, Cancer Center Support Grant P30, Phase II I/UCRC University of California-Davis: Center for Biophotonics Sensors and Systems
OpenAlex ID: W2904480402