Silica nanoparticle-mediated, Electric field-triggered sensitivity enhancement strategy for Capillary electrophoretic detection of proteins
Year: 2016
Type: article
Abstract: We report in this paper the improved capillary electrophoresis (CE)-UV detection sensitivity of proteins based on silica nanoparticle-mediated, electric field-triggered (SNM-EFT) strategy.During the process, the capillary was first filled with acidic separation buffer, followed by injection of a segment of alkaline treating solution containing silica nanoparticles (SNPs).After the inlet end of the capillary was dipped into the protein solution containing SNPs, a high voltage was applied across the capillary.The SNPs in the protein solution became aggregate, promoting the SNP-protein conjugation, by which transformation of the protein structures took place.During CE, the unfolded protein desorbed from SNPs, generating stronger signal than the native one due to the more effective exposure of tryptophanyl residues to the polar buffer.The parameters of the treatment protocols, e.g., pH of the treating solution, concentration of the SNPs presented, the voltage and duration of the electric field applied, had notable effects on the detection sensitivity.Significant improvement in CE-UV response was obtained by the EFT at -8 kV × 60 s on the protein standards dissolved in 4 mM sodium tetraborate, 1 mM boric acid and 0.015% SNPs.
Authors: Aimin Wang, Weidong Qin
Institution: Beijing Normal University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2905580209