Year: 2019
Type: article
Abstract: To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activity at cellular resolution is needed. Here, we introduce wavelength multiplexing to perform fast volumetric two-photon imaging of cortical columns (>2,000 neurons in 10 planes at 10 vol/s), using two different calcium indicators, an electrically tunable lens and a spatial light modulator. We image the activity of neuronal populations from layers 2/3 to 5 of primary visual cortex from awake mice, finding a lack of columnar structures in orientation responses and revealing correlations between layers which differ from trial to trial. We also simultaneously image functional correlations between presynaptic layer 1 axons and postsynaptic layer 2/3 neurons. Wavelength multiplexing enhances high-speed volumetric microscopy and can be combined with other optical multiplexing methods to easily boost imaging throughput.
Source: Cell Reports
Authors: Shuting Han, Weijian Yang, Rafael Yuste
Institution: Columbia University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,200
Funders: Howard Hughes Medical Institute, Burroughs Wellcome Fund, National Institutes of Health, Multidisciplinary University Research Initiative, National Institute of Mental Health, National Eye Institute, National Institute of Neurological Disorders and Stroke, Army Research Office, Army Research Laboratory
Awards: G1242600127, G1480873338, G2723399458, G4012432327, G5259331294, Imaging Functional Connectivity in Visual Cortex, CRCNS: Modeling the nanophysiology of dendritic spines, Functional connectomics of the neocortical microcircuit, G7452299184, Effect of GABAergic inhibition of dendritic spines on synaptic pruning in the medial prefrontal cortex during adolescence
OpenAlex ID: W2946736675