Work

BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture

Year: 2015

Type: article

Abstract: The study was undertaken with a view to establish a protocol for in vitro plant regeneration from shoot tip explants of banana.Different concentrations of BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/l) and IBA (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) was used in MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation of Amritasagar and Sabri banana cultivars.Maximum single shoot formation (50.0% and 30.0%), number of single shoot (3.50 and 2.00 in), longest shoot (2.64 cm and 2.16 cm) were found from 0.5 mg/l BAP while maximum number of roots (3.83 and 2.50) and longest root (3.60 cm and 3.10 cm) was found from 0.3 mg/l IBA in Amritasagar and Sabri respectively.The survival of the plantlets of both cultivars was more than 82% under ex vitro condition.0.5 mg/l BAP and 0.3 mg/l IBA can be used with MS media for shoot and root formation of Amritasagar and Sabri banana cultivars through shoot tip culture.

Source: Journal of Bioscience and Agriculture Research

DOI: 10.18801/jbar.030215.35

Authors: M. H. Ferdous, A. A. Masum Billah, H. Mehraj, T. Taufique, A. F. M. Jamal Uddin

Institutions: Sher-e-Bangla Agricultural University, Ehime University

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

OpenAlex ID: W2907013063