Work

Kinetic Analysis of Hepatic Metabolism Using Hyperpolarized Dihydroxyacetone

Year: 2019

Type: article

Abstract: Hyperpolarized carbon-13 magnetic resonance (HP-MR) is a new metabolic imaging method the does not use ionizing radiation. Due to the inherent chemical specificity of MR, not only tracer uptake but also downstream metabolism of the agent is detected in a straightforward manner. HP [2- 13 C] dihydroxyacetone (DHA) is a promising new agent that directly interrogates hepatic glucose metabolism. DHA has three metabolic fates in the liver: glucose production, glycerol production and potential inclusion into triglycerides, and oxidation in the tricarboxylic acid cycle. Each pathway is regulated by flux through multiple enzymes. Using Duhamel’s formula, the kinetics of DHA metabolism is modeled, resulting in estimates of specific reaction rate constants. The multiple enzymatic steps that control DHA metabolism make more simplified methods for extracting kinetic data less than satisfactory. The described modeling paradigm effectively identifies changes in metabolism between gluconeogenic and glycogenolytic models of hepatic function.

Source: Journal of Chemical Information and Modeling

DOI: 10.1021/acs.jcim.8b00745

Authors: Alexander Kirpich, Mukundan Ragavan, James A. Bankson, Lauren M. McIntyre, Matthew E. Merritt

Institutions: University of Florida, The University of Texas MD Anderson Cancer Center, The University of Texas Health Science Center at Houston

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green