Work

Emulsion Agglutination Assay for the Detection of Protein–Protein Interactions: An Optical Sensor for Zika Virus

Year: 2019

Type: article

Abstract: A new class of Janus emulsion agglutination assay is reported for the detection of interfacial protein-protein interactions. Janus emulsion droplets are functionalized with a thermally stable, antigen binding protein rcSso7d variant (rcSso7d-ZNS1) for the detection of Zika NS1 protein. The emulsion droplets containing fluorescent dyes in their hydrocarbon and fluorocarbon phases intensify the intrinsic optical signal with the emission intensity ratio, which can be detected by a simple optical fiber. This assay provides an opportunity for the in-field detection of Zika virus and other pathogens with a stable, inexpensive, and convenient device.

Source: ACS Sensors

DOI: 10.1021/acssensors.8b01202

Authors: Qifan Zhang, Lukas Zeininger, Ki-Joo Sung, Eric A. Miller, Kosuke Yoshinaga, Hadley D. Sikes, Timothy M. Swager

Institutions: Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Singapore-MIT Alliance for Research and Technology Centre, Deutsche Forschungsgemeinschaft, Deshpande Center for Technological Innovation, Massachusetts Institute of Technology, Tata Center for Technology and Design, Massachusetts Institute of Technology, National Cancer Institute, National Institute of General Medical Sciences, Division of Graduate Education

Awards: G3360732593, G4456013424, G5662436926, G7787715925, Molecules for Dynamic Nuclear Polarization and NMR Structure Determination, Cancer Center Support (Core) Grant

OpenAlex ID: W2910147525

FWCI: 2.729

Cites: 15

Cited by: 41

Related to: 10