Exosomes Derived from Human Primed Mesenchymal Stem Cells Induce Mitosis and Potentiate Growth Factor Secretion
Year: 2019
Type: article
Abstract: Mesenchymal stem cells (MSCs) facilitate functional recovery in numerous animal models of inflammatory and ischemic tissue-related diseases with a growing body of research suggesting that exosomes mediate many of these therapeutic effects. It remains unclear, however, which types of proteins are packaged into exosomes compared with the cells from which they are derived. In this study, using comprehensive proteomic analysis, we demonstrated that human primed MSCs secrete exosomes (pMEX) that are packaged with markedly higher fractions of specific protein subclasses compared with their cells of origin, indicating regulation of their contents. Notably, we found that pMEX are also packaged with substantially elevated levels of extracellular-associated proteins. Fibronectin was the most abundant protein detected, and data established that fibronectin mediates the mitogenic properties of pMEX. In addition, treatment of SHSY5Y cells with pMEX induced the secretion of growth factors known to possess mitogenic and neurotrophic properties. Taken together, our comprehensive analysis indicates that pMEX are packaged with specific protein subtypes, which may provide a molecular basis for their distinct functional properties.
Source: Stem Cells and Development
Authors: Oliver Yuan, Clayton Lin, Joseph Wagner, Joseph S. Anderson, Joehleen A. Archard, Peter Deng, Julian Halmai, Gerhard Bauer, Kyle D. Fink, Brian Fury, Nicholas H. Perotti, Jon E. Walker, Kari Pollock, Michelle Apperson, Janelle Butters, Peter Belafsky, D. Gregory Farwell, Maggie Kuhn, Jan Nolta, Johnathon D. Anderson
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Research Resources, National Center for Advancing Translational Sciences
Awards: Training Program in Biomolecular Technology, Predoctoral Training in Pharmacological Sciences, West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC), Direct cell to cell transfer of microRNA for tissue repair, Shared LSRII Cytometer Equipment Application - UC Davis Stem Cell Program, UC Davis Clinical and Translational Science Center, Training Program in Basic and Translational Cardiovascular Science, Cancer Center Support Grant P30, Institutional Career Development Core (KL2)
OpenAlex ID: W2910190385