Work

Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes

Year: 2018

Type: review

Abstract: Genetically engineered mouse models are commonly preferred for studying the human disease due to genetic and pathophysiological similarities between mice and humans.In particular, Cre-loxP system is widely used as an integral experimental tool for generating the conditional.This system has enabled researchers to investigate genes of interest in a tissue/cell (spatial control) and/or time (temporal control) specific manner.A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed.This review provides a brief overview of Cre-loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase.Also, we finally introduce some available links to the Web sites that provides detailed information about Cre mouse lines including their characterization.

Source: Laboratory Animal Research

DOI: 10.5625/lar.2018.34.4.147

Authors: Hyeonhui Kim, Minki Kim, Sun-Kyoung Im, Sungsoon Fang

Institutions: Yonsei University, Yonsei University Health System

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Research Foundation, Yonsei University, National Research Foundation of Korea, Yonsei University College of Medicine

Awards: G1582815025, G5329673206, G7332546408