Tracking Neural Progenitor Cell Migration in the Rodent Brain Using Magnetic Resonance Imaging
Year: 2019
Type: review
Abstract: The study of neurogenesis and neural progenitor cells (NPCs) is important across the biomedical spectrum, from learning about normal brain development and studying disease to engineering new strategies in regenerative medicine. In adult mammals, NPCs proliferate in two main areas of the brain, the subventricular zone and the subgranular zone, and continue to migrate even after neurogenesis has ceased within the rest of the brain. In healthy animals, NPCs migrate along the rostral migratory stream (RMS) from the subventricular zone to the olfactory bulb, and in diseased animals, NPCs migrate towards lesions such as stroke and tumors. Here we review how MRI-based cell tracking using iron oxide particles can be used to monitor and quantify NPC migration in the intact rodent brain, in a serial and relatively non-invasive fashion. NPCs can either be labeled directly in situ by injecting particles into the lateral ventricle or RMS, where NPCs can take up particles, or cells can be harvested and labeled in vitro, then injected into the brain. For in situ labeling experiments, the particle type, injection site, and image analysis methods have been optimized and cell migration toward stroke and multiple sclerosis lesions has been investigated. Delivery of labeled exogenous NPCs has allowed imaging of cell migration towards more sites of neuropathology, which may enable new diagnostic and therapeutic opportunities for as-of-yet untreatable neurological diseases.
Source: Frontiers in Neuroscience
Institution: Michigan State University
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,950
Funders: Michigan State University, National Institutes of Health, College of Engineering, Michigan State University, National Institute on Aging, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering
Awards: G104518089, (PQC5) MRI of magnetically labeled immune/stem cells for early tumor detection, G2115346025, G2848036350, G4740530596, MRI-based immune cell tracking in Alzheimer?s disease, G4870124175, G549440430, Molecular Imaging for Detection of Synthetic Biology Circuits, Oscillators and Toggle Switches in Regenerative Medicine, Quantitative molecular and cellular MRI of hepatocyte transplantation, MRI Contrast Agents for In vivo Monitoring of Stem Cell Differentiation
OpenAlex ID: W2910941309