Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish
Year: 2019
Type: article
Abstract: Long-term monitoring of intrinsic electrocardiogram (ECG) in zebrafish plays a crucial role in heart disease studies as well as drug screening. In this work, we developed a polymer-based apparatus with embedded flexible thin-film electrodes to acquire ECG signals of awake zebrafish. The apparatus was made of polydimethylsiloxane (PDMS) using the molding technique with molds formed by 3D printing. A graphical user interface (GUI) was built in National Instruments LabView platform for real-time recording, processing and analysis. The program provided important features, such as signal de-noising, characteristic wave detection and anomaly detection. Further, it could operate on both real-time coming signals as well as previously-saved data, facilitating analysis and interpretation. We demonstrated the use of our system to investigate the effects of the anesthetic drug, namely Tricaine (MS-222), on cardiac electrophysiology of zebrafish, revealing promising findings. We speculate that our novel system may contribute to a host of studies in various disciplines using the zebrafish model.
Source: IEEE Sensors Journal
Authors: Tai Le, Michael Lenning, Isaac Clark, Ishaan Bhimani, Joseph Fortunato, Paul Marsh, Xiaolei Xu, Hung Cao
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Awards: CAREER: Wireless Flexible Micro-sensors to Monitor Cardiac Phenotypes in Zebrafish Models of Heart Regeneration, Micro-Sensors to Study Electrical and Mechanical Coupling of Injured Myocardium, Genetic Studies of Sarcomere-based Cardiac Diseases, Epicardial remodeling in cardiomyopathy and cardiac aging
OpenAlex ID: W2912148688