Work

Axially-offset differential interference contrast microscopy via polarization wavefront shaping

Year: 2019

Type: article

Abstract: Sample-scan phase contrast imaging was demonstrated by producing and coherently recombining light from a pair of axially offset focal planes. Placing a homogeneous medium in one of the two focal planes enables quantitative phase imaging using only common-path optics, recovering absolute phase without halo or oblique-illumination artifacts. Axially offset foci separated by 70 μm with a 10x objective were produced through polarization wavefront shaping using a matched pair of custom-designed microretarder arrays, compatible with retrofitting into conventional commercial microscopes. Quantitative phase imaging was achieved by two complementary approaches: i) rotation of a half wave plate, and ii) 50 kHz polarization modulation with lock-in amplification for detection.

Source: Optics Express

DOI: 10.1364/oe.27.003837

Authors: Changqin Ding, Chen Li, Fengyuan Deng, Garth J. Simpson

Institutions: Purdue University West Lafayette, Boston University

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: Purdue University, Purdue Research Foundation, National Institutes of Health, National Institute of General Medical Sciences