Work

Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications

Year: 2018

Type: conference-paper

Abstract: Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.

Source: IEEE International Conference on Nano/Molecular Medicine and Engineering (NANOMED)

DOI: 10.1109/nanomed.2018.8641558

Authors: Timothy S. Frost, Linan Jiang, Yitshak Zohar

Institution: University of Arizona

Language: English

Topic: Barrier Structure and Function Studies

Subfield: Neurology

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Aeronautics and Space Administration, Arizona Biomedical Research Commission