Work

Handheld line-scanned dual-axis confocal microscope with pistoned MEMS actuation for flat-field fluorescence imaging

Year: 2019

Type: article

Abstract: A handheld line-scanned dual-axis confocal (LS-DAC) microscope has been developed for high-speed (16 frames/s) fluorescence imaging of tissues with sub-nuclear resolution. This is the first miniature fluorescence LS-DAC system that has been fully packaged for handheld clinical use on patients. A novel micro-electro-mechanical system scanning mechanism, with synchronized tilting and pistoning, is used to achieve flat-field en face imaging. We show that this facilitates video mosaicking to generate images that sample an extended lateral field of view.

Source: Optics Letters

DOI: 10.1364/ol.44.000671

Authors: Linpeng Wei, Chengbo Yin, Yoko Fujita, Nader Sanai, Jonathan T. C. Liu

Institutions: University of Washington, Barrow Neurological Institute

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of Dental and Craniofacial Research

Awards: Line-scanned MEMS-based dual-axis confocal microscopy to detect oral lesions in v, Intraoperative confocal microscopy for quantitative delineation of low-grade glio