Year: 2018
Type: conference-paper
Abstract: This work is the first demonstration of a 3D printed, modular, multimodal electrochemical-sensor-integrated, transwell system for monitoring tissue culture and molecular signaling, in vitro.Integral to the design of this platform is a porous membrane hosting an in vitro tissue culture with cell-interfaced electrochemical sensors.Interfacial -electrical cell surface impedance sensing (ECIS) electrodes and -cyclic voltammetric electrodes (CV) are used to monitor epithelial barrier formation and basolateral molecular release respectively.Such interfacial sensor-integrated tissue culture platform enables unprecedented access and provides temporal information that conventional transwells cannot furnish.We use this platform to uncover signaling events occurring along the gutmicrobiome-brain-axis (GMBA).
Institution: University of Maryland, College Park
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2913921057