Work

OMIP‐052: An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques

Year: 2019

Type: article

Abstract: This 18-color panel was designed to measure vaccine-induced T cell responses in rhesus macaques by intracellular cytokine staining (ICS).It detects seven cytokines, IFN-γ, IL-2, IL-4, IL-5, IL-13, IL-17, and IL-21, two memory markers, CD45RA and CCR7, and four follicular helper T cell (Tfh) markers, CXCR3, CXCR5, ICOS, and PD-1 (Figure 1).While this panel was optimized for use on cryopreserved rhesus macaque peripheral blood mononuclear cells (PBMC) (Table 1), it can also be used on rhesus macaque tissue samples and human PBMC and tissue samples.

Source: Cytometry Part A

DOI: 10.1002/cyto.a.23670

Authors: Mitzi M. Donaldson, Shing‐Fen Kao, Kathryn E. Foulds

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases, Vaccine Research Center

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases