Work

Acid Stress Triggers Resistance to Acetic Acid-Induced Regulated Cell Death throughHog1Activation Which RequiresRTG2in Yeast

Year: 2019

Type: article

Abstract: Acid stress causes resistance to acetic acid-induced regulated cell death (AA-RCD) in budding yeast, resulting in catalase activation. In order to explore the molecular determinants of evasion of AA-RCD triggered by acid stress adaptation, we studied the involvement and the possible interplay of the master regulator of transcription high-osmolarity glycerol 1 (HOG1) andRTG2, a positive regulator of theRTG-dependent mitochondrial retrograde signaling. Viability, DNA fragmentation, and ROS accumulation have been analyzed in wild-type and mutant cells lackingHOG1and/orRTG2. Catalase activity and transcription ofCTT1andCTA1, coding the cytosolic and peroxisomal/mitochondrial catalase, respectively, as well asHog1phosphorylation, were also analyzed. Our results show thatHOG1is essential for resistance to AA-RCD and its activation results in the upregulation ofCTT1, but notCTA1, transcription during acid stress adaptation.RTG2is required forHog1-dependentCTT1upregulation upon acid stress, despite failure ofRTGpathway activation. We give evidence thatRtg2has a cytoprotective role and can act as a general cell stress sensor independent of Rtg1/3-dependent transcription.

Source: Oxidative Medicine and Cellular Longevity

DOI: 10.1155/2019/4651062

Authors: Nicoletta Guaragnella, Mariarita Stirpe, Domenico Marzulli, Cristina Mazzoni, Sergio Giannattasio

Institutions: Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, University of Bari Aldo Moro, Istituto Pasteur, Sapienza University of Rome

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,680

Funder: European Regional Development Fund