Year: 2019
Type: article
Abstract: Despite the link between adverse birth outcomes due to pre- and peri-natal nicotine exposure, research suggests 11% of US women continue to smoke or use alternative nicotine products throughout pregnancy. Maternal smoking has been linked to incidence of craniofacial anomalies. We hypothesized that pre-natal nicotine exposure may directly alter craniofacial development independent of the other effects of cigarette smoking. To test this hypothesis, we administered pregnant C57BL6 mice drinking water supplemented with 0, 50, 100 or 200 μg/ml nicotine throughout pregnancy. On postnatal day 15 pups were sacrificed and skulls underwent micro-computed tomography (µCT) and histological analyses. Specific nicotinic acetylcholine receptors, α3, α7, β2, β4 were identified within the calvarial growth sites (sutures) and centers (synchondroses). Exposing murine calvarial suture derived cells and isotype cells to relevant circulating nicotine levels alone and in combination with nicotinic receptor agonist and antagonists resulted in cell specific effects. Most notably, nicotine exposure increased proliferation in calvarial cells, an effect that was modified by receptor agonist and antagonist treatment. Currently it is unclear what component(s) of cigarette smoke is causative in birth defects, however these data indicate that nicotine alone is capable of disrupting growth and development of murine calvaria.
Source: Scientific Reports
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,790
Funders: U.S. Department of Health and Human Services, U.S. Department of Veterans Affairs, Plastic Surgery Foundation, Medical University of South Carolina, University of South Carolina, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Dental and Craniofacial Research, National Center for Advancing Translational Sciences
Awards: The effects of pharmacological exposures on cranial suture stem cells, G2486139536, G2688050934, G3314368649, G3578994178, G39381196, South Carolina Clinical & Translational Research Institute (SCTR), G4287604158, G5379980822, G559739151, G6113202117, Direct Effects of Nicotine on Cranial Growth and Development, G8002900575, G8533035296, MUSC Center for Oral Health Research, G8953398868, G9000086239, T-COHR: Training in Craniofacial and Oral Health Research
OpenAlex ID: W2918779466