Year: 2014
Type: review
Abstract: This review summarizes the historical and more recent developments of multiphoton microscopy, as applied to dermatology. Multiphoton microscopy offers several advantages over competing microscopy techniques: there is an inherent axial sectioning, penetration depths that compete well with confocal microscopy on account of the use of near-infrared light, and many two-photon contrast mechanisms, such as second-harmonic generation, have no analogue in one-photon microscopy. While the penetration depths of photons into tissue are typically limited on the order of hundreds of microns, this is of less concern in dermatology, as the skin is thin and readily accessible. As a result, multiphoton microscopy in dermatology has generated a great deal of interest, much of which is summarized here. The review covers the interaction of light and tissue, as well as the various considerations that must be made when designing an instrument. The state of multiphoton microscopy in imaging skin cancer and various other diseases is also discussed, along with the investigation of aging and regeneration phenomena, and finally, the use of multiphoton microscopy to analyze the transdermal transport of drugs, cosmetics and other agents is summarized. The review concludes with a look at potential future research directions, especially those that are necessary to push these techniques into widespread clinical acceptance. Keywords: Multiphoton microscopy; skin; cancer; aging and regeneration
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Funders: National Science Foundation, Wellcome Trust, National Research Foundation, National Research Foundation Singapore, Skolkovo Institute of Science and Technology, National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering, Division of Chemical, Bioengineering, Environmental, and Transport Systems
Awards: A Versatile, Minimally-Invasive Treatment for Cancer: Applying Temporal Focusing to Photodynamic Therapy., G270120649, G281903038, G4035684527, G4113606792, G4473449232, NSF Science and Technology Center: Emergent Behaviors of Integrated Cellular Systems, MIT Laser Biomedical Research Center (P41-RR02594), High Throughput Manufacturing for Three-Dimensional Microfluidic Devices, A Versatile, Minimally-Invasive Treatment for Cancer: Applying Temporal Focusing to Photodynamic Therapy., G9000091012, G9000283576
OpenAlex ID: W2920915285