Work

Effect of the total galactose content on complement-dependent cytotoxicity of the therapeutic anti-CD20 IgG1 antibodies under temperature stress conditions

Year: 2019

Type: article

Abstract: The degree of monoclonal antibody galactosylation is known to affect complement-dependent cytotoxicity (CDC) activity by affecting C1q binding, suggesting that galactose is associated with CDC bioactivity. However, whether this association also exists under temperature stress conditions is not known. This study highlights the impact of variations in the terminal galactose content of an anti-CD20 monoclonal antibody on CDC bioactivity under high-temperature stress conditions compared with storage conditions at 2–8 °C. Drug product samples with a total galactose content of >38% showed stable CDC bioactivity at higher temperatures (45 °C), while those with 16% galactose content showed reduced CDC activity.

Source: Journal of Carbohydrate Chemistry

DOI: 10.1080/07328303.2018.1541995

Authors: Bala Reddy Bheemareddy, Mallikarjuna Pulipeta, Pradeep Iyer, Vijaya R. Dirisala

Institution: Vignan's Foundation for Science, Technology & Research

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2921183353

FWCI: 0.398

Cites: 34

Cited by: 7

Related to: 10