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Which Antioxidant System Shapes Intracellular H 2 O 2 Gradients?

Year: 2019
Type: other
Abstract: Cellular antioxidant systems control the levels of hydrogen peroxide (H 2 O 2 ) within cells. Multiple theoretical models exist that predict the diffusion properties of H 2 O 2 depending on the rate of H 2 O 2 generation and amount and reaction rates of antioxidant machinery components. Despite these theoretical predictions, it has remained unknown how antioxidant systems shape intracellular H 2 O 2 gradients. The relative role of thioredoxin (Trx) and glutathione systems in H 2 O 2 pattern formation and maintenance is another disputed question. Here, we visualized cellular antioxidant activity and H 2 O 2 gradients formation by exploiting chemogenetic approaches to generate compartmentalized intracellular H 2 O 2 and using the H 2 O 2 biosensor HyPer to analyze the resulting H 2 O 2 distribution in specific subcellular compartments. Using human HeLa cells as a model system, we propose that the Trx system, but not the glutathione system, regulates intracellular H 2 O 2 gradients. Antioxid. Redox Signal. 31, 664–670.
DOI: 10.1089/ars.2018.7697
Authors: Natalie M. Mishina, Yulia A. Bogdanova, Yulia G. Ermakova, Anastasiya S. Panova, Daria A. Kotova, Dmitry S. Bilan, Benjamin Steinhorn, Elias S.J. Arnér, Thomas Michel, Vsevolod V. Belousov
Institutions: Institute of Bioorganic Chemistry, European Molecular Biology Laboratory, Brigham and Women's Hospital, Harvard University, Karolinska Institutet, Pirogov Russian National Research Medical University, University of Göttingen
Language: English
Open Access status: green
OpenAlex ID: W2921215264
Cites: 9
Cited by: 66
Related to: 10