Work

Escherichia coli as a platform for the study of phosphoinositide biology

Year: 2019

Type: article

Abstract: -inositol, incorporate phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PI4P), phosphatidylinositol-4,5-bisphosphate (PIP2), and phosphatidylinositol-3,4,5-trisphosphate (PIP3) into their plasma membrane. We tested models of biophysical mechanisms with these phosphoinositides in a living membrane, using our system to evaluate the role of PIP2 in nonconventional protein export of human basic fibroblast growth factor 2. We found that PI alone is sufficient for the process.

Source: Science Advances

DOI: 10.1126/sciadv.aat4872

Authors: Sergio Botero, Rachel Chiaroni-Clarke, Sanford M. Simon

Institution: Rockefeller University

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $4,500

Funder: National Cancer Institute