Ex Vivo Expansion of Cord Blood-Derived Endothelial Cells Using a Novel Xeno-Free Culture Media
Year: 2019
Type: article
Abstract: Aim: Endothelial cells (ECs), isolated from peripheral blood (PB), bone marrow (BM) and cord blood (CB), are limited in numbers and expansion has had limited success. We used a novel serum-free medium (EndoGo) to evaluate effects on ex vivo expansion of CB-derived ECs. Materials & methods: Flow cytometry and matrigel were used to determine expansion of ECs and for determination of the EC progenitor cell. Results: EndoGo™-containing cultures demonstrated superior expansion and stimulated proliferation of two distinct subpopulations, CD34+CD31+ and CD34-CD31+, which exhibited different morphology, phenotype and function. EndoGo also expanded the CB endothelial progenitor cells from freshly isolated CB. Conclusion: These findings demonstrate the potential of EndoGo to expand CB ECs, which could generate increased numbers of ECs for therapeutic applications.
Source: Future Science OA
Institution: The University of Texas MD Anderson Cancer Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,165
OpenAlex ID: W2923686382