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Developing chemically modified redox-responsive proteins as smart therapeutics

Year: 2019
Type: article
Abstract: The conditional control of protein function in response to the physiological changes of diseased cells is essential to develop smart protein therapeutics. Herein, we report a redox-responsive chemical modification of a protein by conjugating an intracellular glutathione (GSH)-cleavable ligand, NSA, onto a protein residue. We demonstrated that the NSA conjugation of Ribonuclease A (RNase A) enabled the control of the protein function by GSH in an aqueous solution and living cells, with extended applications for targeted cancer therapy using a lipid nanoparticle-based intracellular protein delivery strategy.
DOI: 10.1039/c9cc00519f
Authors: Qiao Tang, Jianxue Wang, Ying Jiang, Meining Zhang, Jin Chang, Qiaobing Xu, Lanqun Mao, Ming Wang
Institutions: Renmin University of China, Hunan University, Chinese Academy of Sciences, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, University of Chinese Academy of Sciences, Tufts University
Language: English
Open Access status: green
OpenAlex ID: W2925677621
FWCI: 0.992
Cites: 28
Cited by: 16
Related to: 10