Year: 2008
Type: conference-paper
Abstract: Incorporation of spiral continuous flow (CF) polymerase chain reaction (PCR) devices into a 96-well titer plate format will enable highly parallel analyses of many nucleic acids under steady-state temperature control for the realization of a high throughput CFPCR multi-reactor platform.Prior to realization of the full platform, verifying the feasibility of small footprint CFPCR devices, each device confined to a footprint of 8x8 mm 2 , throughout the manufacturing and amplification processes was essential.Small footprint, CFPCR devices in a CFPCR multi-reactor module were designed and double-side hot embossed on one side of a substrate and structures for thermal isolation on the other to improve thermal management.Successful amplification of 99 bp target DNA fragments from λ-DNA template was demonstrated in the small footprint, 20-and 25-turn CFPCR devices.
Authors: D.S. Park, P.-C. Chen, B.H. You, N. Kim, T. Park, T.Y. Lee, P. Datta, Y. Desta, S.A. Soper, D.E. Nikitopoulos, M.C. Murphy
Institutions: Louisiana State University, BioFluidica (United States)
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2928131550