Work

Recombinant Viral Vectors as Neuroscience Tools

Year: 2019

Type: review

Abstract: Recombinant viruses are highly efficient vehicles for in vivo gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans-synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno-associated viruses, retro/lentiviruses, and glycoprotein-deleted rabies viruses. © 2019 by John Wiley & Sons, Inc.

Source: Current Protocols in Neuroscience

DOI: 10.1002/cpns.67

Authors: Shih‐Heng Chen, Juhee Haam, Mitzie Walker, Erica Scappini, John Naughton, Negin P. Martin

Institutions: National Institutes of Health, National Institute of Environmental Health Sciences, United States Department of Health and Human Services, Salk Institute for Biological Studies

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Institute of Environmental Health Sciences