Year: 2014
Type: conference-paper
Abstract: We present a real-time multi-experiment biofilm analysis platform using a valve-actuated microfluidic system, designed to reduce growth variance of in-vitro biofilms.After 24 hours of biofilm growth in the microfluidic device the biofilms exhibited only a 9% variation in biomass, showing significantly reduced deviation in comparison to the biomass variation seen in our previous work conducted in separate microfluidic channels.A linear array charge-coupled device enabled real-time tracking of both average biomass and localized morphology by monitoring the change in optical density.We demonstrate the multi-experiment capability of the microfluidic device and the spatiotemporal monitoring capacity of the system by tracking the growth of biofilms when subjected to different treatments.
Institution: University of Maryland, College Park
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2930850873