Work

A Nanostructured Impedance Microsensor for Real-time Monitoring of Macromolecular Assembly and Elisa-on-a-chip

Year: 2014

Type: conference-paper

Abstract: This paper presents an impedimetric microsensor that monitors nano sensing probe functionalization in real-time to optimize on-chip enzyme-linked immunosorbent assays (ELISA).The self-assembly dynamics of Tobacco mosaic virus-like particle (VLP) bioreceptors were studied using continuous measurement of the electrical impedance between the interdigitated microelectrodes, which contributed to an optimized and reduced VLP assembly time from 18 hours to 3 hours.The impedance sensor with optimized VLP assembly effectively sensed antibody binding in on-chip ELISA which showed a 64% higher impedance change compared to the control experiment.These results combined highlight the significant potential of genetically modified VLPs as selective nanostructured probes for rapid immunoassays.

Source: Solid-State Sensors, Actuators, and Microsystems Workshop (Hilton Head Workshop)

DOI: 10.31438/trf.hh2014.47

Authors: F. Zang, X.Z. Fan, K. Gerasopoulos, L. Naves, J. Culver, R. Ghodssi

Institution: University of Maryland, College Park

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funders: National Science Foundation, Division of Civil, Mechanical and Manufacturing Innovation, Army Research Office

Awards: G2952877981, Nanofabrication Using Viral Biotemplates for MEMS Applications, G4346593422