Displacement analysis of myocardial mechanical deformation (DIAMOND) reveals segmental susceptibility to doxorubicin-induced injury and regeneration
Year: 2019
Type: article
Abstract: Zebrafish are increasingly utilized to model cardiomyopathies and regeneration. Current methods evaluating cardiac function have known limitations, fail to reliably detect focal mechanics, and are not readily feasible in zebrafish. We developed a semiautomated, open-source method - displacement analysis of myocardial mechanical deformation (DIAMOND) - for quantitative assessment of 4D segmental cardiac function. We imaged transgenic embryonic zebrafish in vivo using a light-sheet fluorescence microscopy system with 4D cardiac motion synchronization. Our method permits the derivation of a transformation matrix to quantify the time-dependent 3D displacement of segmental myocardial mass centroids. Through treatment with doxorubicin, and by chemically and genetically manipulating the myocardial injury-activated Notch signaling pathway, we used DIAMOND to demonstrate that basal ventricular segments adjacent to the atrioventricular canal display the highest 3D displacement and are also the most susceptible to doxorubicin-induced injury. Thus, DIAMOND provides biomechanical insights into in vivo segmental cardiac function scalable to high-throughput research applications.
Source: JCI Insight
Institutions: University of California, Los Angeles, Mayo Clinic
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,100
Awards: Electrochemical Impedance Spectroscopy to Assess Metabolically Active Plaque, Linking Spatial Variations in Shear Stress with Oxidation Stress, Shear stress and light-field to elucidate the initiation of cardiac outflow tract, Micro-Sensors to Study Electrical and Mechanical Coupling of Injured Myocardium, Genetic Studies of Sarcomere-based Cardiac Diseases, Epicardial remodeling in cardiomyopathy and cardiac aging
OpenAlex ID: W2939359575