Work

Development of ergosterol peroxide probes for cellular localisation studies

Year: 2019

Type: article

Abstract: Ergosterol peroxide selectively exhibits biological activity against a wide range of diseases; however, its mode of action remains unknown. Here, we present an efficient synthesis of ergosterol peroxide chemical probes for in vitro anticancer evaluation, live cell studies and proteomic profiling. Ergosterol peroxide analogues show promising anti-proliferation activity against triple negative breast cancer cellular models, revealing information on the structure-activity relationship of this natural product in order to develop superior analogues. The combined cellular studies demonstrate that ergosterol peroxide is distributed across the cytosol with significant accumulation in the endoplasmic reticulum (ER). These chemical probes support our efforts towards uncovering the potential target(s) of ergosterol peroxide against triple negative breast cancer cell lines.

Source: Organic & Biomolecular Chemistry

DOI: 10.1039/c9ob00145j

Authors: Taotao Ling, Walter H. Lang, Michelle M. Martinez-Montemayor, Fatima Rivas

Institutions: St. Jude Children's Research Hospital, Central University of the Caribbean

Language: English

Topic: Natural product bioactivities and synthesis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid