Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications
Year: 2019
Type: article
Abstract: Utilizing polymers in cardiac tissue engineering holds promise for restoring function to the heart following myocardial infarction, which is associated with grave morbidity and mortality. To properly mimic native cardiac tissue, materials must not only support cardiac cell growth but also have inherent conductive properties. Here, we present an injectable reverse thermal gel (RTG)-based cardiac cell scaffold system that is both biocompatible and conductive. Following the synthesis of a highly functionalizable, biomimetic RTG backbone, gold nanoparticles (AuNPs) were chemically conjugated to the backbone to enhance the system's conductivity. The resulting RTG-AuNP hydrogel supported targeted survival of neonatal rat ventricular myocytes (NRVMs) for up to 21 days when cocultured with cardiac fibroblasts, leading to an increase in connexin 43 (Cx43) relative to control cultures (NRVMs cultured on traditional gelatin-coated dishes and RTG hydrogel without AuNPs). This biomimetic and conductive RTG-AuNP hydrogel holds promise for future cardiac tissue engineering applications.
Authors: Brisa Peña, Marcos Maldonado, Andrew J. Bonham, Brian A. Aguado, Antonio Dominguez-Alfaro, Melissa Laughter, Teisha J. Rowland, James Bardill, Nikki L. Farnsworth, Nuria Alegret Ramon, Matthew R. G. Taylor, Kristi S. Anseth, Maurizio Prato, Robin Shandas, Timothy A. McKinsey, Daewon Park, Luisa Mestroni
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: green
Awards: Development of a nanotherapy for aortic valve stenosis, Whole genome DNA sequencing to identify novel genes in dilated cardiomyopathy, G241012502, G2451848122, G2635073672, Screening and Development of Small Molecule HDAC11 Inhibitors to Treat Obesity and Diabetes., Genetics, Mechanisms and Clinical Phenotypes of Arrhythmogenic Cardiomyopathy, Deacetylase-Dependent Control of Diastolic Dysfunction and HFpEF, G3528081209, Regulation of Cardiac Signaling by Class I Histone Deacetylases, Comprehensive Clinical Assessment of Pediatric PHT, Molecular Genetics, Pathogenesis and Protein-Replacement Therapy in Arrhythmogenic Cardiomyopathy, Epigenetic Readers as Downstream Effectors of Beta1-Adrenergic Receptor Signaling in HFrEF, G5760946491, Regulation of Chromatin Signaling in Heart Failure by the BRD4 Bromodomain Protein., G6681927405, G707899701, G7683404682, G7875213309, G8067420242, Functional and Biological Phenotyping of Pediatric PH, A biomimetic reverse thermal gel for the treatment of myocardial infarction, Engineered biomimetic injectable system towards cardiac repair
OpenAlex ID: W2942007485