Year: 2019
Type: preprint
Abstract: We present a new type of photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent isomerization followed by protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we created probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
DOI: 10.1101/626853
Institutions: Max Planck Institute for Medical Research, École Polytechnique Fédérale de Lausanne, Heidelberg University, European Molecular Biology Laboratory, European Molecular Biology Laboratory, European Molecular Biology Laboratory, European Molecular Biology Laboratory, European Molecular Biology Laboratory, NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
Funders: European Molecular Biology Laboratory, École Polytechnique Fédérale de Lausanne, Kommission für Technologie und Innovation, Max-Planck-Gesellschaft, National Center of Competence in Research Chemical Biology, National Institutes of Health, National Institute on Drug Abuse, National Institute of Biomedical Imaging and Bioengineering, H2020 European Research Council, Common Fund
Awards: Reconstructing the dynamic 3D architecture of the human genome by superresolution microscopy and DNA sequence modelling., G4875136982, Structural cell biology in situ using superresolution microscopy, Reconstructing the dynamic 3D architecture of the human genome by superresolution microscopy and DNA sequence modelling.
OpenAlex ID: W2943065156